A novel alkaline and low-temperature lipase of Burkholderia cepacia isolated from Bohai in China for detergent formulation

作者：Wang HaiKuan^*; Liu RuiJuan; Lu FuPing; Qi Wei; Shao Jing; Ma HuiJing

来源：Annals of Microbiology, 2009, 59(1): 105-110.

DOI：10.1007/bf03175606

• Burkholderia cepacia
• alkaline lipase
• low-temperature lipase
• purification
• detergent

The bacterial strain LP08 was isolated from soil collected from bay of Bohai, China. The sequence of 16S rDNA of strain LP08 showed 99% homology to Burkholderia cepacia. The lipase from Burkholderia cepacia LP08 was purified by ammonium sulphate precipitation, ion exchange chromatography and Sephadex G-75 chromatography. The characterization of the lipase exhibited maximum activity at 30 degrees C and pH 9.0. The lipase retained 63, 66, 74, and 95% of its maximum activity at 10, 15, 20 and 25 degrees C respectively. The lipase activity was promoted in the presence of commercial detergent, sodium cholate, sodium taurocholate, glycerine and NaCl, while was little inhibited in the presence of TritonX-100, Tween-20, Tween-80, SDS, saponin. The present lipase was highly stable towards oxidizing agents and was stable after 1 h at 25 degrees C in the presence of hydrogen peroxide, sodium hypochlorite and sodium perborate. The results suggest that the lipase from Burkholderia cepacia LP08 showed good potential for application in the detergent formulation.

• 出版日期2009
• 单位天津科技大学