A New Primer Set to Amplify the Mitochondrial Cytochrome C Oxidase Subunit I (COI) Gene in the DHA-Rich Microalgae, the Genus Aurantiochytrium

作者：Nishitani Goh^*; Yoshida Masaki

来源：Microbes and Environments, 2018, 33(2): 227-229.

DOI：10.1264/jsme2.ME17145

• Aurantiochytrium
• mitochondrial primer
• COI

This study was performed in order to develop a primer set for mitochondria! cytochrome c oxidase subunit I (COI) in the DHA-rich microalgae of the genus Aurantiochytrium. The performance of the primer set was tested using 12 Aurantiochytrium strains and other thraustochytrid species. There were no genetic polymorphisms in the mitochondria! sequences from the Aurantiochytrium strains, in contrast to the nuclear 18S rRNA gene sequence. This newly developed primer set amplified sequences from Aurantiochytrium and closely related genera, and may be useful for species identification and clarifying the genetic diversity of Aurantiochytrium in the field.

• 出版日期2018-7-4