A rapid and sensitive chemiluminescent immunoassay of total thyroxin with DMAE center dot NHS-labeled

作者：Yin, Dongguang; Cui, Daxiang; Gao, Feng; He, Rong; He, Youfeng; Liu, Yibing; Shen, Decun; Wu, Minghong^*

来源：Journal of Immunoassay and Immunochemistry, 2008, 29(3): 257-265.

DOI：10.1080/15321810802119075

• chemiluminescence immunoassay
• DMAE center dot NHS
• streptvaidin-biotin
• total thyroxin

A novel chemiluminescence immunoassay (CLIA) of total thyroxin (TT4) with the acridinium ester of DMAE center dot NHS [2',6'-dimethyl-4'-(n-succinimidyloxycarbonyl) phenyl-10-methyl-acridinium-9-carboxylate methosulfate] labeled has been developed. In our method, microwells were coated with anti-T4 monoclonal antibody (McAb) and DMAE center dot NHS synthesized by our laboratory was conjugated with streptavidin (SA). T4-BSA was conjugated with biotin-N-hydroxysuccinimide (B center dot NHS), T4-BSA- B center dot NHS and the T4 in the standard or sample competitively react with anti-T4 McAb. Streptavidin-biotin separation and enhanced techniques were applied in this assay. The presented approach shows many excellent characteristics, particularly, rapid assay process and high detection sensitivity.

• 出版日期2008
• 单位中国原子能科学研究院; 上海交通大学; 上海大学