Application of chitosan for wound repair in dogs.

作者：Inas N El Husseiny^*; Kawkab A Ahmed

来源：Life Science Journal-Acta Zhengzhou University Overseas Edition, 2012, 9(1): 196-203.

• chitosan
• chitin
• shell products
• biomaterials

This experimental work was applied to study the effect of topical application of chitosan powder on the stimulation of healing of full thickness skin wounds. Experimental surgical wounds were done in 12 apparently healthy male Mongrel dogs of nearly the same age and weight. Experimental animals were classified into 4 groups each consisted of 3 dogs according to time of euthanasia. Full thickness equal longitudinal skin incision wounds were created on each dog%26apos;s both side at the dorsal aspect of the animal. A comparative study was applied between wounds treated with chitosan powder and control wounds washed only by saline solution. Wound healing was clinically evaluated during the period of the experiment. Euthanasia was done at different period intervals, one week, two weeks, three weeks and four weeks after incisions. Specimen were taken for histopathological investigations. Results proved rapid regeneration and reepithelization of the wounds treated with chitosan powder compared with those of the control group. Cinically, complete healing was seen after 3 weeks in the chitosan treated wounds which delayed to 4 weeks in the control ones. Histopatholgical investigations proved presence of more pronounced granulation tissues in the chitosan treated wounds than in the control ones. Healing started at 3 weeks post- incision in the treated group and complete repair was achieved at four weeks. Complete regeneration of epidermal cells with keratin layer occurred which was similar to the normal skin associated with dermal connective tissue proliferation. The fibroblast cells laid down a network of collagen fibers which appeared as wavy collagen bundles surrounding the neovasculature of the wounds, whereas, in control group, hyalinosis of subcutaneous granulation tissue and haphazardly arranged collagen fibers were observed. In conclusion, chitosan proved to be a suitable biomedical agent used for the acceleration of wound repair due to its biocompatibility, easy application and high effectiveness. %26lt;br%26gt;[Inas, N.El-Husseiny and Kawkab, A. Ahmed. Application of chitosan for wound repair in dogs. Life Science Journal, 2012; 9(1): 196-203] (ISSN: 1097-8135). http://www.lifesciencesite.com. 28

• 出版日期2012