Developing a biomaterial interface based on poly(lactic acid) via plasma-assisted covalent anchorage of D-glucosamine and its potential for tissue regeneration

作者：Swilem Ahmed E; Lehocky Marian^*; Humpolicek Petr; Kucekova Zdenka; Junkar Ita; Mozetic Miran; Hamed Ashraf A; Novak Igor

来源：Colloids and Surfaces B: Biointerfaces , 2016, 148: 59-65.

DOI：10.1016/j.colsurfb.2016.08.046

• D-Glucosamine
• Carbodiimide
• Poly(lactic acid)
• Plasma treatment
• Fibroblast viability

The aim of this study was to develop the potential tissue engineering applications of e-glucosamine (GlcN) immobilized onto the surface of a biodegradable matrix in order to induce a desired biological effect at biointerfaces. Thus, for sample preparation we used a novel multistep physicochemical approach. In the first step the poly(lactic acid) (PLA) films were exposed to a low pressure plasma in air atmosphere, followed by radical graft copolymerization with acrylic acid to yield a carboxyl-functionalized spacer layer on the PLA surface. The carboxyl groups were then coupled to GlcN molecules via the carbodiimide chemistry. The developed surfaces were characterized by X-ray Photoelectron Spectroscopy (XPS), Contact angle measurements and Atomic Force Microscopy (AFM). A preliminary study on the proliferation of fibroblasts on the developed surfaces was performed using the NIH/3T3 cell line.

• 出版日期2016-12-1