Measurement of atropine and scopolamine in hair by LC-MS/MS after Datura stramonium chronic exposure

作者：Ricard Florian; Abe Emuri; Duverneuil Mayer Charlotte; Charlier Philippe; de la Grandmaison Geoffroy; Alvarez Jean Claude^*

来源：Forensic Science International, 2012, 223(1-3): 256-260.

DOI：10.1016/j.forsciint.2012.09.015

• Datura stramonium
• Atropine
• Scopolamine
• LC/MS/MS
• Hair

Background: Datura stramonium is an herbaceous annual plant. All parts of the plant contain tropane alkaloids such as atropine and scopolamine. We report the case of a 22-year-old man admitted to a general hospital for visual and aural hallucinations. One week after his admission, as the hallucinations remained, the patient was transferred to a psychiatric hospital. Neither blood nor urine was conserved during his hospitalization, so a hair analysis was requested in order to identify a possible consumption of a Datura seed infusion. %26lt;br%26gt;Methods: After decontamination and washing, hair strands were segmented into four pieces and grinded into a fine and homogeneous powder. We then incubated 20 mg for 10 min in 1 mL of phosphate buffer at pH 5.0 in the presence of 100 ng of ketamine-d4, used as internal standard (IS). Liquid-liquid extraction was performed with 4 mL of a mixture of hexane/ethyl acetate (1/1, v/v). The residue was reconstituted in 80 mu L of mobile phase. A further 10 mu L were injected into an 1.9 mu m Hypersil GOLD PFP column (100 mm x 2.1 mm) eluted with a gradient of acetonitrile and 2 mmol/L 0.1% formate buffer at a flow rate of 300 mu L/min. Compounds were detected by a LCQ TSQ Vantage XP triple-quadripole mass spectrometer equipped with an electrospray ionization (ESI) source set in positive mode. SRM transitions m/z 290.2 -%26gt; 124.1, m/z 304.2 -%26gt; 138.1, and m/z 242.1 -%26gt; 129.1 were optimized for atropine, scopolamine and IS, respectively. %26lt;br%26gt;Results: The assay was accurate and precise over the range of 1.0 (lower limit of quantification) to 1000.0 pg/mg (upper limit of quantification) in hair. Both atropine (from 8.4 to 15.0 pg/mg) and scopolamine (1.0-1.3 pg/mg) were identified in the four segment of the hair showing a regular consumption of Datura admitted by the patient himself. %26lt;br%26gt;Conclusion: We report here the first description of atropine with scopolamine in a Caucasian dark man%26apos;s hair after D. stramonium chronic exposure, using a validated LC-MS/MS method.

• 出版日期2012-11-30