科研之友
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摘要
Anthocyanins are mostly plant pigments responsible for red, blue, and purple colors. These are distributed in agricultural products. In addition, the various functional activities of anthocyanins have been studied, such as antioxidant, antiobesity, antidiabetic, and so on. Thus, it is important to analyze the compositions of anthocyanins in edible fruits or vegetables. Nuclear magnetic resonance (NMR) is often used for chemical structure determinations of organic compounds, but recently has been used for molecular species analysis. In this study, the anthocyanins in 13 kinds of berries collected from various regions were qualified and quantified. They were then compared with the components in berries using multivariate statistics on H-1-NMR spectra, and their antioxidant activity by oxygen radical absorbance capacity (ORAC) assay was evaluated. Concerning the H-1-NMR spectra, each anthocyanin of cyanidin and pelargonidin glycosides was identified, and the contents were evaluated using the specific signals at around delta(H) 8.80-9.20. Principal components analysis (PGA) using the H-1-NMR spectra revealed that 13 kinds of berries can be clearly distributed according to their contents of anthocyanins. The antioxidant activity of each berry using the ORAC assay was shown as 20-70 mu mol Trolox equivalent (TE)/g of fresh fruit. The activity was found to be correlated to the contents of anthocyanins (r= 0.72). Furthermore, the principal component 1 (PC1) value from PCA was strongly correlated (r= 0.86). PC1 showed the amount of cyanidin-3-glycoside. Thus, the antioxidant activity in berries was correlated with the content of cyanidin-3-glycoside. This NMR-based molecular species analysis method may be applied to the controling the quality of products and the screening of unique types of berries.
- 出版日期2016-6
