Nonsense-mutated inlA and prfA not widely distributed in Listeria monocytogenes isolates from ready-to-eat seafood products in Japan

作者：Handa Miya Satoko; Kimura Bon^*; Takahashi Hajime; Sato Miki; Ishikawa Tatsuya; Igarashi Kazunori; Fujii Tateo

来源：International Journal of Food Microbiology, 2007, 117(3): 312-318.

DOI：10.1016/j.ijfoodmicro.2007.05.003

• Listeria monocytogenes
• internalin A
• ready-to-cat
• seafood

InIA is a surface protein participating in the entry of Listeria monocytogenes into mammalian non-phagocytic cells. PrfA is a positive regulatory factor that regulates the expression of a set of virulence genes. Recent studies revealed that some L. monocytogenes strains have a truncated form of these proteins because of nonsense mutations in their sequences, and these truncations contribute to the significant reduction in virulence of this pathogen. In this study, sequence analyses of inIA and prfA among L. monocytogenes isolated from ready-to-eat seafood revealed that only one out of 59 isolates had a nonsense-mutated MIA and all had non-mutated prfA. This indicated that these strains could be fully virulent based on the sizes of these proteins.

• 出版日期2007-7-15