A fluorescence lifetime spectrometer based on a synchroscan streak camera

作者：Shao, Yong-Hong; Li, Heng; Wang, Yan; Qu, Jun-Le; Niu, Hanben^*

来源：Journal of Shenzhen University Science and Engineering, 2009, 26(4): 331-336.

• Fluorescence decay profile
• Fluorescence dyes
• Fluorescence lifetimes
• Nonlinear least square fitting
• Sweep speed
• Temporal resolution
• Two photon excitation fluorescence
• Two-photon excitations

A two-photon excitation fluorescence lifetime spectrometer based on a synchroscan streak camera was presented. The spectrometer was calibrated using a Titanium: Sapphire femtosecond laser in conjunction with a custom-made synchronized streak circuit, tunable delay generator and a set of etalons. Experimental results show that the temporal resolution of this spectrometer is 9 ps, nonlinearity is less than 4%, and the sweep range is 2.8 ns. Fluorescence decay profile of fluorescent dye Rose Bengal (RsB) is recorded with the spectrometer. Nonlinear least square fitting of the decay profile gives that the lifetime of RsB is 763 ps, consistent with standard fluorescence dyes.

• 出版日期2009