Determination of Salmonella Enteritidis FT 13 A and Salmonella Issatschenko by PCR in samples of chicks experimentally infected

作者：Monroy Becerra Israel; Ledesma Martinez Nestor; Sanchez Godoy Felix Domingo; Ruiz Flores Griselda; Urquiza Bravo Odette

来源：Veterinaria Mexico, 2012, 43(4): 257-271.

• CLASSIC PCR
• NESTED PCR
• SALMONELLA ENTERITIDIS
• SALMONELLA ISSATSCHENKO
• CHICKS

The objective of this work was to determine by PCR the presence of Salmonella Enteritidis FT-13A (SE) and Salmonella Issatschenko (SI) in different samples of organs from 4 days old chicks experimentally infected. Four days old chicks were inoculated with SE and SI and their organs were frozen for DNAextraction to perform classic and nested PCR. It was possible to demonstrate the presence of SE and SI after 6 hours after experimental infection (AEI), but as time passed AEI positive results were inconsistent, obtaining negative results until 174 hours AEI. PCR is useful for detecting SE and SI in the early hours AEI. It is recommended to use pre-enrichment of the samples, in order to facilitate the DNA extraction and the detection of Salmonella by PCR.

• 出版日期2012-12