Accelerated discovery via a whole-cell model

作者：Sanghvi Jayodita C; Regot Sergi; Carrasco Silvia; Karr Jonathan R; Gutschow Miriam V; Bolival Benjamin Jr; Covert Markus W^*

来源：Nature Methods, 2013, 10(12): 1192-+.

DOI：10.1038/NMETH.2724

To test the promise of whole-cell modeling to facilitate scientific inquiry, we compared growth rates simulated in a whole-cell model with experimental measurements for all viable single-gene disruption Mycoplasma genitalium strains. Discrepancies between simulations and experiments led to predictions about kinetic parameters of specific enzymes that we subsequently validated. These findings represent, to our knowledge, the first application of whole-cell modeling to accelerate biological discovery.

• 出版日期2013-12