A superoxide anion generator, pyrogallol, inhibits the growth of HeLa cells via cell cycle arrest and apoptosis

作者：Kim Sang Wook; Han Yong Whan; Lee Soo Teik; Jeong Hey Jin; Kim Seong Hun; Kim In Hee; Lee Seung Ok; Kim Dae Ghon; Kim Suhn Hee; Kim Sung Zoo; Park Woo Hyun^*

来源：Molecular Carcinogenesis, 2008, 47(2): 114-125.

DOI：10.1002/mc.20369

• pyrogallol
• ROS
• cell cycle
• apoptosis
• HeLa
• GSH

We investigated the in vitro effects of pyrogallol on cell growth, cell cycle regulation, and apoptosis in HeLa cells. Pyrogallol inhibited the growth of HeLa cells with an IC50 of approximately 45 mu M. Pyrogallol induced arrest during all phases of the cell cycle and also very efficiently resulted in apoptosis in HeLa cells, as evidenced by flow cytometric detection of sub-G1 DNA content, annexin V binding assay, and DAPI staining. This apoptotic process was accompanied by the loss of mitochondrial transmembrane potential (Delta Psi(m)), Bcl-2 decrease, caspase-3 activation, and PARP cleavage. Pan-caspase inhibitor (Z-VAD) could rescue some HeLa cells from pyrogallol-induced cell death, while caspase-8 and -9 inhibitors unexpectedly enhanced the apoptosis. When we examined the changes of the ROS, H2O2 or O-2(-) in pyrogallol-treated cells, H2O2 was slightly increased and O-2(-) significantly was increased. in addition, we detected a decreased GSH content in pyrogallol-treated cells. Only pan-caspase inhibitor showing recovery of GSH depletion and reduced intracellular O-2(-) level decreased PI staining in pyrogallol-treated HeLa cells, which indicates dead cells. in summary, we have demonstrated that pyrogallol as a generator of ROS, especially O-2(-), potently inhibited the growth of HeLa cells through arrests during all phases of the cell cycle and apoptosis.

• 出版日期2008-2