Detection of herpesvirus variant (OsHV-1 mu var) in Pacific oysters (Crassostrea gigas) in Spain and development of a rapid method for its differential diagnosis

作者：Aranguren R; Costa M M; Novoa B; Figueras A^*

来源：Bulletin of the European Association of Fish Pathologists, 2012, 32(1): 24-29.

Ostreid herpesvirus-1 (OsHV-1) infections have been reported around the world associated with mortalities of the Pacific oyster, Crassostrea gigas. Recently, a new genotype called OsHV-1 mu var was described associated with important mortalities of C. gigas in France and has been considered a dangerous emerging pathogen for oysters.
In the present work we describe the presence of this new genotype OsHV-1 mu var in Spain associated to mortalities of C. gigas. Moreover, we have developed a specific polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay to identify OsHV-1 mu var and distinguish it from the reference herpesvirus genotype. We propose this technique as a useful method to provide a rapid identification of the OsHV-1 mu var in epizootiological studies and disease control programmes.

• 出版日期2012