A sensitive LC-ESI-MS/MS method for the quantification of avobenzone in rat plasma and skin layers: Application to a topical administration study

作者：Kim Min Gi; Kim Tae Hwan; Shin Beom Soo; Kim Min Gyu; Seok Su Hyun; Kim Kyu Bong; Lee Jong Bong; Choi Hyeon Gwan; Lee Young Sung; Yoo Sun Dong^*

来源：Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 2015, 1003: 41-46.

DOI：10.1016/j.jchromb.2015.09.014

• Avobenzone
• LC-MS/MS
• Absorption
• Sunscreen

This study describes the development of a sensitive high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) method for the quantification of avobenzone in rat plasma and skin layers. Separations were performed on a Zorbax SB C-8 column using a binary gradient mobile phase composed of acetonitrile and 0.1% formic acid in water. The assay achieved LLOQ of 0.5 ng/ml for plasma, 5 ng/ml for stratum corneum, and 10 ng/ml for epidermis and dermis. This method was applied to a percutaneous absorption study of avobenzone in rats. At 12 h following topical application of emulsion and lotion (applied amount of avobenzone 11.7 mg/kg), avobenzone was found primarily in the stratum corneum (16.3-17.8%) followed by epidermis (2.0-3.4%) and dermis (0.11-0.15%). Avobenzone was not quantifiable in the plasma samples collected over a 12 h sampling period. Given the excellent plasma assay sensitivity, this study provides evidence that the systemic absorption of avobenzone is insignificant, if any, after topical application.

• 出版日期2015-10-15