Mini-Tn7 vectors for studying post-transcriptional gene expression in Pseudomonas

作者：Liu Yunhao; Rainey Paul B; Zhang Xue Xian^*

来源：Journal of Microbiological Methods, 2014, 107: 182-185.

DOI：10.1016/j.mimet.2014.10.015

• Gene regulation
• LacZ
• Noncoding RNAs
• Translational fusion
• Mini-Tn7
• Pseudomonas

We describe the construction and validation of five mini-Tn7 vectors for analysis of post-transcriptional gene expression in Pseudomonas. Four vectors allow construction of translational fusions to beta-galactosidase (lacZ), while the fifth is designed for functional analysis of noncoding RNA genes. Translational fusions can be constructed without a functional promoter in the vector or from an inducible promoter of either P-tac or P-dctA. We show that promoterless fusions have value for determining levels of translation, whereas fusions to inducible promoters have utility in the analysis of mRNA-binding factors.

• 出版日期2014-12