A selective fluorescent probe for relay detection of Zn2+ and tartrate: Application to logic circuit and living cell imaging

作者：Yao, Kun; Fu, Jiaxin; Chang, Yongxin; Li, Bai; Yang, Li; Xu, Kuoxi^*

来源：Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy , 2018, 205: 410-418.

DOI：10.1016/j.saa.2018.07.051

• Zn2+
• Tartrate anion
• Relay fluorescent detection
• Logic circuit
• Cell imaging

A novel fluorescent probe 2-(2'-hydroxyphenyl)-4-(phenylethylamino)methyloxazole (HPO) has been synthesized, which performed highly selective and sensitive detection of Zn2+ ion with a discriminating enhancement over the other metal ions. The binding constant was calculated as 3.07 x 10(3) M-1 with detection limit of 1.22 x 10(-6) M in aqueous solution (CH3CN-Tris v/v, 1/1, Tris, 10 mM, pH = 7.4). Moreover, the HPO-Zn2+ complex could serve as an excellent tartrate anion (TA). The detection mode maybe due to TA detach Zn2+ ion from HPO-Zn2+ complex leading to resulting in the release of the free probe HPO. As a result, a logic circuit has also been constructed on the basis of Zn2+ and TA as chemical inputs. Furthermore, fluorescence imaging experiments showed that probe HPO could be used as an effective fluorescent probe for detecting Zn2+ and TA in living cells.

• 出版日期2018-12-5
• 单位河南大学