A nonsense mutation in a putative sulphate transporter gene results in low phytic acid in barley

作者：Ye, Hongxia; Zhang, Xiao-Qi; Broughton, Sue; Westcott, Sharon; Wu, Dianxing^*; Lance, Reg; Li, Chengdao

来源：Functional & Integrative Genomics, 2011, 11(1): 103-110.

DOI：10.1007/s10142-011-0209-4

• Comparative mapping
• Candidate gene
• Rice
• Barley
• HvST

Low phytic acid grains can provide a solution to dietary micronutrient deficiency and environmental pollution. A low phytic acid 1-1 (lpa1-1) barley mutant was identified using forward genetics and the mutant gene was mapped to chromosome 2HL. Comparative genomic analysis revealed that the lpa1-1 gene was located in the syntenic region of the rice Os-lpa-MH86-1 gene on chromosome 4. The gene ortholog of rice Os-lpa-MH86-1 (designated as HvST) was isolated from barley using polymerase chain reaction and mapped to chromosome 2HL in a doubled haploid population of ClipperxSahara. The results demonstrate the collinearity between the rice Os-lpa-MH86-1 gene and the barley lpa1-1 region. Sequence analysis of HvST revealed a single base pair substitution (C -> T transition) in the last exon of the gene in lpa1-1 (M422), which resulted in a nonsense mutation. These results will facilitate our understanding of the molecular mechanisms controlling the low phytic acid phenotype and assist in the development of a diagnostic marker for the selection of the lpa1-1 gene in barley.

• 出版日期2011-3
• 单位湖州市农业科学研究院; 浙江大学