New limits of sensitivity of site-directed spin labeling electron paramagnetic resonance for membrane proteins

作者：Bordignon Enrica^*; Bleicken Stephanie

来源：Biochimica et Biophysica Acta-Biomembranes, 2018, 1860(4): 841-853.

DOI：10.1016/j.bbamem.2017.12.009

• EPR
• Nitroxide
• Spectroscopy
• Dynamics
• Water accessibility
• Distances

Site-directed spin labeling electron paramagnetic resonance is a biophysical technique based on the specific introduction of spin labels to one or more sites in diamagnetic proteins, which allows monitoring dynamics and water accessibility of the spin-labeled side chains, as well as nanometer distances between two (or more) labels. Key advantages of this technique to study membrane proteins are addressed, with focus on the recent developments which will expand the range of applicability. Comparison with other biophysical methods is provided to highlight the strength of EPR as complementary tool for structural biology. This article is part of a Special Issue entitled: Beyond the Structure-Function Horizon of Membrane Proteins edited by Ute Hellmich, Rupak Doshi and Benjamin Mcllwain.

• 出版日期2018-4