科研之友
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摘要
Histamine is a neurotransmitter and chemical mediator in multiple physiological processes. Histamine H-3 receptor is expressed in the nervous system, heart, and gastrointestinal tract; however, little is known about H-3 receptor in skeletal muscle. The aim of this study was to investigate the role of H-3 receptor in skeletal myotubes. The expression of H-3 receptor and myosin heavy chain (MHC), a late myogenesis marker, was assessed by real-time PCR and immunostaining in C2C12 skeletal myogenesis and adult mid-urethral skeletal muscle tissues. H-3 receptor mRNA showed a significant increase upon differentiation of C2C12 into myotubes: 1-, 26-, 91-, and 182-fold at days 0, 2, 4, and 6, respectively. H-3 receptor immunostaining in differentiated C2C12 cells and adult skeletal muscles was positive and correlated with that of MHC. The functional role of H(3)receptor in differentiated myotubes was assessed using an H-3 receptor agonist, (R)-a-methylhistamine ((R)-alpha-MeHA). Ca2+ imaging, stimulated by electric pacing, was decreased by 55% after the treatment of mature C2C12 myotubes with 1 mu M (R)-alpha-MeHA for 10 min and 20 min, while treatment with 100 nm (R)-alpha-MeHA for 5 min caused 45% inhibition. These results suggested that H-3 receptor may participate in the maintenance of the relaxed state and prevention of over-contraction in mature differentiated myotubes. The elucidation of the role of H3R in skeletal myogenesis and adult skeletal muscle may open a new direction in the treatment of skeletal muscle disorders, such as muscle weakness, atrophy, and myotonia in motion systems or pen-urethral skeletal muscle tissues.
- 出版日期2015-5-5
- 单位郑州大学
