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摘要
Background: Biofilm formation on indwelling devices is one of the most important mechanisms playing a role in device-related urinary tract infections caused by Proteus mirabilis. Increasing antibiotic resistance of microorganisms has raised questions concerning the relationship between biofilm formation and drug resistance.
Objective: To determine clinical prevalence, antibiotic resistance of, and biofilm formation by P. mirabilis strains.
Methods: We studied the susceptibilities of various P. mirabilis strains isolated from different clinical materials by a disc-diffusion method. Biofilm formation was determined by a crystal violet binding assay.
Results: Two (13%) of 15 P. mirabilis strains were found to be strong biofilm formers (SBF). Both SBF P. mirabilis strains were isolated from urine samples from children less than 15 years old in a pediatric emergency unit. Cefixime, cefazolin, ceftriaxone, amikacin, and piperacillin/tazobactam were the most effective antibiotics against 15 P. mirabilis strains (100%), whereas SBF P. mirabilis strains were multidrug resistant or (resistant to 5 different antimicrobial classes). Both of the SBF P. mirabilis strains, but neither of the weak biofilm forming P. mirabilis strains were resistant to ampicillin and ceftazidime among beta-lactam antibiotics, or tobramycin and gentamicin among aminoglycoside antibiotics used in the present study.
Conclusions: Children comprise the only patients infected with SBF P. mirabilis strains and both SBF P. mirabilis strains displayed high antimicrobial resistance in our setting.
- 出版日期2015-8
