Diurnal variation and effect of insulin on circulating high molecular weight (HMW) adiponectin and NF-kappa B activity in human endothelial cells

作者：Tan Bee K^*; Adya Raghu; Lewandowski Krzysztof C; O'Hare J Paul; Randeva Harpal S

来源：Atherosclerosis, 2011, 214(1): 174-177.

DOI：10.1016/j.atherosclerosis.2010.10.013

• Adipokine
• Adiponectin
• Atherosclerosis
• Diabetes
• NF-kappa B

Objective: To study the diurnal variation and the effect of insulin on adiponectin multimers and nuclear factor-kappaB (NF-kappa B) activity in human endothelial cells.
Methods and results: We utilized a prolonged insulin-glucose infusion in six healthy human subjects. HMW and total adiponectin levels were higher in the morning and lower at night; NF-kappa B activities in serum treated human microvascular endothelial cells (HMEC-1) cells were lower in the morning and higher at night. Hyperinsulinemic induction significantly decreased HMW and total adiponectin levels but increased NF-kappa B activity in serum treated HMEC-1 cells (P < 0.05, P < 0.01). There were no significant changes to MMW and LMW adiponectin levels (P > 0.05).
Conclusion: Circadian rhythm of HMW adiponectin and NF-kappa B activity are altered by hyperinsulinemia providing novel insights adiponectin and NF-kappa B biology, which may be pertinent to insulin resistant states, e. g. obesity and type 2 diabetes mellitus.

• 出版日期2011-1