Antimicrobial resistance and pulsed-field gel electrophoresis typing of Vibrio parahaemolyticus isolated from shrimp mariculture environment along the east coast of China

作者：Zhao, Shu; Ma, Licai; Wang, Yuan; Fu, Guihong; Zhou, Junfang; Li, Xincang; Fang, Wenhong^*

来源：Marine Pollution Bulletin, 2018, 136: 164-170.

DOI：10.1016/j.marpolbul.2018.09.017

• Vibrio parahaemolyticus
• Antimicrobial resistance
• PFGE
• Food safety
• Mariculture farms

Environmental antimicrobial resistance (AMR) has drawn increasing attention due to its great risk to human health. The aim of this study was to investigate AMR and genotyping of Vibrio parahaemolyticus isolates (n = 114) recovered from shrimp mariculture environment in China. The isolates exhibited a high rate of resistance to streptomycin (78.9%), ampicillin (64.9%) and gentamicin (53.5%). Furthermore, multi-drug resistance was highly prevalent (61.4%), in which 95.9% of these ampicillin-resistant isolates were primarily mediated by bla(CARB-17). Surprisingly, doxycylcine, florfenicol, and trimethoprim/sulfamethoxazole (TMP/SMZ) resistance genes occurred in susceptible isolates. Moreover, 114 isolates were grouped into unique pulsed field gel electrophoresis patterns. These findingssuggest the need for the prudent use of antimicrobial agents on mariculture farms, in order to control the dissemination of antimicrobial resistant V. parahaemolyticus.

• 出版日期2018-11
• 单位同济大学; 中国水产科学研究院; 中国农业大学