Different organic components on silica hybrid matrices modulate the lipase inhibition by the glycerol formed in continuous transesterification reactions

作者：Martin Lucas S; Ceron Annie; Oliveira Pedro C; Zanin Gisella M; de Castro Heizir F^*

来源：Journal of Industrial and Engineering Chemistry, 2018, 62: 462-470.

DOI：10.1016/j.jiec.2018.01.029

• Lipase
• Hybrid matrices
• Glycerol affinity
• Lipase immobilization
• Transesterfficadon

Silica hybrid materials, functionalized via incorporation of three organic components: beta-cyclodextrin (beta CD), carboxymethyl-cellulose (CMC) and hydroxyethyl-cellulose (HEC) were synthesized by the sol-gel technique and results were compared to a well-defined matrix (silica-polyvinyl alcohol-SiO2-PVA) with respect to immobilizing Burkholderia cepacia lipase. The main objective was set to select organic components that can replace the PVA to obtain a hybrid composite with greater hydrophobic character, without compromising the remarkable features of SiO2-PVA. All the three selected compounds allowed obtaining matrices that presented similar textural and morphological properties and gave high activity (1451-1661 U g(-1)) and thermal stability (t(1/2) > 70 h) upon immobilization. Regarding glycerol affinity, all matrices had lower ability than SiO2-PVA to adsorb glycerol, with the SiO2-beta CD matrix showing the lowest affinity due to the cyclic structure of the beta CD. Transesterification reactions of palm kernel oil with ethanol mediated by B. cepacia immobilized on SiO2-beta CD performed in a packed bed reactor under continuous flow confirmed the efficiency of the SiO2-beta CD matrix in increasing the operational stability of system, revealing a half-life (t(1/2) = 1040 h), 2.5 times greater than that achieved in the same system using lipase immobilized on SiO2-PVA (t(1/2) = 430 h).

• 出版日期2018-6-25