AN ANALYSIS OF THE METABOLISM OF Aspergillus niger GROWING OVER A SOLID SUBSTRATE

作者：Reyes Ocampo I; Gonzalez Brambila M; Lopez Isunza F^*

来源：Revista Mexicana de Ingenieria Quimica, 2013, 12(1): 41-56.

• Aspergillus niger metabolism
• structured model
• continuous measurements of CO2 and O-2
• modified Petri dish
• biomass growth on solid substrate

The metabolism of Aspergillus niger A10 growing on agar plates is analyzed using a structured model that includes the pathways for glicolysis (EMP), pentose-phosphate (PP), the Krebs cycle (TCA), and mycelial biosynthesis; and takes into account enzyme regulation in EMP and TCA either due to low or high levels of citrate and ATP in cytosol. Model predictions are supported by a series of experiments measuring mycelial growth under different glucose concentrations in the agar plates, as well as the use of a two-carbon sources mixtures. Besides measurements of biomass, CO2 production and O-2 consumption were also continuously measured in the gas-phase of a modified Petri dish. Atkinson's energy charge (1969) concept is used to analyze the growth efficiency under increasing glucose concentration in the agar plates in the range: 25 to 250 gL(-1).

• 出版日期2013-4