科研之友
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摘要
Objective. To evaluate the effects of krill oil (KO) on inflammation and redox status in dextran sulfate sodium (DSS)-induced colitis in rats. Materials and methods. Thirty male Wistar rats were divided into three groups: Control, DSS, and DSS + KO 5% in a 4-week diet study. Colitis was induced by 5% DSS in the drinking water the last week of the experiment. Weight and disease activity index (DAI), colon length, histological combined score (HCS), colon levels of selected cytokines and prostaglandins, markers of protein oxidative damage, fatty acid profile, and expression of selected genes were measured. Results. Rats in the DSS group increased their DAI and HCS compared with healthy controls. The colon length was significantly preserved after KO diet. Tumor necrosis factor (TNF)-alpha and interleukin (IL)-1 beta were elevated in the DSS group compared with controls. Cytokines and HCS were nonsignificantly lower in the KO versus the DSS group. Prostaglandin (PG) E-3 increased significantly in the KO versus the other groups. Peroxisome proliferator-activated receptor (PPAR)-gamma expression was nonsignificantly increased while PPAR-gamma coactivator 1 alpha (Pparg1 alpha) expression increased significantly after KO. The levels of protein oxidation markers decreased significantly. Conclusions. KO showed protective potential against DSS colitis based on the preservation of colon length, reduction of oxidative markers and the consistent beneficial changes of HCS, cytokine, and (PG)E-3 levels, as well as PPAR-gamma and Pparg1 alpha expression compared with DSS alone. These findings indicate an anti-inflammatory and a protein antioxidant effect of KO.
- 出版日期2012-1
- 单位河北医科大学
