Upregulation and secretion of macrophage inhibitory cytokine-1 (MIC-1) in gastric cancers

作者：Baek Kyoung Eun; Yoon Suk Ran; Kim Jong Tae; Kim Kwang Soo; Kang Seong Ho; Yang Young; Lim Jong Seok; Choi Inpyo; Nam Myoung Soo; Yoon Michung; Lee Hee Gu^*

来源：Clinica Chimica Acta, 2009, 401(1-2): 128-133.

DOI：10.1016/j.cca.2008.12.008

• MIC-1
• Macrophage-inhibitory cytokine-1
• GDF-15
• Growth differentiation factor-15
• Gastric cancer

Background: Macrophage inhibitory cytokine-1 (MIC-1), a distant member of the transforming growth factor (TGF)-beta superfamily, has been reported to be upregulated and secreted from several cancers. We examined MIC-1 expression and secretion in gastric cancers.
Methods: MIC-I mRNA and protein levels in cancer tissues and cell lines were analyzed by RT-PCR and Western blot. MIC-1 expression in cancer tissues and its secretion in serum were analyzed using immunohistochemistry and ELISA.
Results: MIC-I was significantly upregulated in gastric cancer tissues and cell lines. MIC-1 was secreted from gastric SNU620 cells and its levels in the serum of cancer patients were 10-fold higher than those of healthy controls. In addition, the staining of MIC-I expression was strongly increased in metastatic gastric cancers.
Conclusions: MIC-I was obviously overexpressed in gastric cancers and MICA secretion into blood may be useful for the prediction of gastric cancer progression.

• 出版日期2009-3