A clinical microchip for evaluation of single immune cells reveals high functional heterogeneity in phenotypically similar T cells

作者：Ma Chao; Fan Rong; Ahmad Habib; Shi Qihui; Comin Anduix Begonya; Chodon Thinle; Koya Richard C; Liu Chao Chao; Kwong Gabriel A; Radu Caius G; Ribas Antoni; Heath James R^*

来源：Nature Medicine, 2011, 17(6): 738-U133.

DOI：10.1038/nm.2375

Cellular immunity has an inherent high level of functional heterogeneity. Capturing the full spectrum of these functions requires analysis of large numbers of effector molecules from single cells. We report a microfluidic platform designed for highly multiplexed (more than ten proteins), reliable, sample-efficient (similar to 1 x 10(4) cells) and quantitative measurements of secreted proteins from single cells. We validated the platform by assessment of multiple inflammatory cytokines from lipopolysaccharide (LPS)-stimulated human macrophages and comparison to standard immunotechnologies. We applied the platform toward the ex vivo quantification of T cell polyfunctional diversity via the simultaneous measurement of a dozen effector molecules secreted from tumor antigen-specific cytotoxic T lymphocytes (CTLs) that were actively responding to tumor and compared against a cohort of healthy donor controls. We observed profound, yet focused, functional heterogeneity in active tumor antigen-specific CTLs, with the major functional phenotypes quantitatively identified. The platform represents a new and informative tool for immune monitoring and clinical assessment.

• 出版日期2011-6