A parallel microfluidic flow cytometer for high-content screening

作者:McKenna Brian K; Evans James G; Cheung Man Ching; Ehrlich Daniel J*
来源:Nature Methods, 2011, 8(5): 401-U41.
DOI:10.1038/NMETH.1595

摘要

A parallel microfluidic cytometer (PMC) uses a high-speed scanning photomultiplier-based detector to combine low-pixel-count, one-dimensional imaging with flow cytometry. The 384 parallel flow channels of the PMCMC decouple count rate from signal-to-noise ratio. Using six-pixel one-dimensional images, we investigated protein localization in a yeast model for human protein misfolding diseases and demonstrated the feasibility of a nuclear-translocation assay in Chinese hamster ovary (CHO) cells expressing an NF kappa B-EGFP reporter.

  • 出版日期2011-5