科研之友
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摘要
Spray-dried plasma (SDP) is a complex mixture of active proteins that modulates the immune response of gut-associated lymphoid tissue, We examined whether SDP and Ig concentrate (IC) supplementation could modulate cytokine expression and inflammatory mediators in rats challenged with Staphylococcus aureus enterotoxin B (SEB). Wistar-Lewis rats were fed diets supplemented with SDP (8% wt:wt), IC (1.5% wt:wt), or milk proteins (control diet) from weaning (d 21) to d 34 after birth. On d 32 and 35, the rats were given SEB (0.5 mg/kg; intraperitoneal). Six hours after the second SEB dose, jejunal mucosa and Peyer's patches (PP) from the small intestine were collected. The cytokines interferon-gamma (IFN gamma), tumor necrosis factor-alpha (TNF alpha), interleukin (IL)-6, IL-10, transforming growth factor-beta (TGF beta), and leukotrienne B(4) (LTB(4)) were analyzed using commercial kits. SEB increased the release of proinflammatory mediators (IFN gamma, TNFa, IL-6, and LTB4) in PIP (P < 0.05) and in the mucosa (P < 0.05). In both tissues, SDP prevented the increase in IFN gamma, IL-6, and LTB4 induced by SEB (P < 0.05). IC reduced the expression of TNFa and LTB4 in PP and mucosa (P < 0.05). SDP supplementation increased IL-10 and mature TGF beta concentrations in intestinal mucosa from both inflamed and noninflamed rats. Both SDP and IC increased the mature:total TGF/3 ratio (all P < 0.05). Both supplements were effective at preventing the SEB-induced increase in proinflammatory:antiinflammatory cytokine ratios in PIP and mucosa and in serum. The preventive effects of plasma supplements on intestinal inflammation involve modulation of intestinal cytokines, characterized by an increased expression of antiinf lammatory cytokines. J. Nutr. 140: 25-30, 2010.
- 出版日期2010-1
