Alpha-phellandrene, a Natural Active Monoterpene, Influences a Murine WEHI-3 Leukemia Model In Vivo by Enhancing Macrophague Phagocytosis and Natural Killer Cell Activity

作者：Lin Jen Jyh; Lu Kung Wen; Ma Yi Shih; Tang Nou Ying; Wu Ping Ping; Wu Chih Chung; Lu Hsu Feng; Lin Jaung Geng; Chung Jing Gung^*

来源：In Vivo, 2014, 28(4): 583-588.

• alpha-phellandrene
• WEHI-3 leukemia cells
• phagocytosis
• natural killer cells
• leukemia Balb/c mice

alpha-phellandrene (alpha-PA), a cyclic monoterpene, is a natural compound reported to promote immune responses in normal BALB/c mice. The effects of alpha-PA on immune responses in a leukemia mouse model were examined. Mice were injected with mouse leukemia WEHI-3 cells and subsequently treated orally with or without alpha-PA (0, 25 and 50 mg/kg) and olive oil as positive control for two weeks. Leukocytes and splenocytes were isolated and cell markers for CD3, CD19, CD11b and Mac-3, phagocytosis and natural killer cell cytoxicity effects were analyzed by flow cytometry. alpha-PA increased the percentage of CD3 (T-cell marker), CD19 (B-cell marker) and MAC3 (macrophages) markers but reduced the percentage of CD11b (monocytes) cell surface markers. alpha-PA (25 and 50 mg/kg) increased phagocytosis of macrophages from blood samples and treatment promoted natural killer cell activity at 25 mg/kg from splenocytes. alpha-PA at 25 mg/kg also increased B- and T-cell proliferation.

• 出版日期2014-8
• 单位中国医科大学