High-speed detection of DNA translocation in nanopipettes

作者:Fraccari Raquel L; Ciccarella Pietro; Bahrami Azadeh; Carminati Marco; Ferrari Giorgio; Albrecht Tim*
来源:Nanoscale, 2016, 8(14): 7604-7611.
DOI:10.1039/c5nr08634e

摘要

We present a high-speed electrical detection scheme based on a custom-designed CMOS amplifier which allows the analysis of DNA translocation in glass nanopipettes on a microsecond timescale. Translocation of different DNA lengths in KCl electrolyte provides a scaling factor of the DNA translocation time equal to p = 1.22, which is different from values observed previously with nanopipettes in LiCl electrolyte or with nanopores. Based on a theoretical model involving electrophoresis, hydrodynamics and surface friction, we show that the experimentally observed range of p-values may be the result of, or at least be affected by DNA adsorption and friction between the DNA and the substrate surface.

  • 出版日期2016