Addressing of LnCaP Cell Using Magnetic Particles Assisted Impedimetric Microelectrode

作者：Dung Thi Xuan Nguyen; Trong Binh Tran; Phuong Diem Nguyen; Min Junhong

来源：Journal of Nanoscience and Nanotechnology, 2016, 16(3): 2933-2936.

DOI：10.1166/jnn.2016.11094

• Patterning
• Metastatic Prostate Tumor Cell
• Proliferation
• Biocompatible
• Magnetic Particle
• Interdigitated
• Impedance Sensing

In this study, we provide a facile, effective technique for a simple isolation and enrichment of low metastatic prostate tumor cell LNCaP using biocompatible, magnetic particles asissted impedimetric sensing system. Hydrophobic cell membrane anchors (BAM) were generated onto magnetic particles which diameters vary from 50 nm to 5 mu m and were used to capture LNCaP cells from the suspension. Finally, magnetic particle-LNCaP complex were addressed onto the surface of the interdigitated microelectrode (IDM). Cell viability was monitored by our laboratory developed-technique Electrical Cell Substrate Impedance Sensing (ECIS). The results reavealed that 50 nm-magnetic particles showed best performance in terms of cell separation and cell viability. This technique provides a simple and efficient method for the direct addressing of LNCaP cell on the surface and enhances better understanding of cell behavior for cancer management in the near future.

• 出版日期2016-3