Novosphingobium piscinae sp nov., isolated from a fish culture pond

作者：Sheu Shih Yi; Chen Zih Han; Chen Wen Ming^*

来源：International Journal of Systematic and Evolutionary Microbiology, 2016, 66(3): 1539-1545.

DOI：10.1099/ijsem.0.000914

A bacterial strain designated SLH-16(T) was isolated from a fish culture pond in Taiwan and characterized using a polyphasic taxonomic approach. Cells of strain SLH-16T were Gram-stain-negative, aerobic, motile rods that were covered by large capsules and formed yellow colonies. Growth occurred at 20-40 degrees C (optimum, 37-40 degrees C), at pH 4.0-9.0 (optimum, pH 5.0-6.0) and with 0-0.5 % (w/v) NaCI (optimum, 0 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain SLH-16T belonged to the genus Novosphingobium and was related most closely to Novosphingobium taihuense T3-B9(T) with sequence similarity of 97.3 5. The major fatty acids (>10 5) of strain SLH-16T were C-18 : (1)omega 7C, summed feature 3 (C-16 :(1)omega 7c and/or C-16 :(1)omega 6c) and C-16 : 0.The major 2-hydroxy fatty acid was C-14 : 0 2 -OH. The polar lipid profile consisted of phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidyldimethylethanolamine, sphingoglycolipid, phosphatidylcholine and several uncharacterized lipids. The major polyamine was spermidine. The major isoprenoid quinone was Q-10. The DNA G+C content was 65.2 mol%. The DNA DNA hybridization value for strain SLH-16T and the type strain of N. taihuense was less than 43.2 %. Phenotypic characteristics of the novel strain also differed from those of the closest related species of the genus Novosphingobium. On the basis of the genotypic, chemotaxonomic and phenotypic data, strain SLH-16T represents a novel species in the genus Novosphingobium, for which the name Novosphingobium piscinae sp. nov. is proposed. The type strain is SLH-16T (=BCRC 80888(T)=LMG 28418(T)=KCTC 42194(T)).

• 出版日期2016-3