Monitoring of Paclitaxel, Taxine B and 10-Deacethylbaccatin III in Taxus baccata L. by Nano LC-FTMS and NMR Spectroscopy

作者：Ghassempour Alireza^*; Rezadoost Hassan; Mashouf Alireza; Aboul Enein Hassan Y; Spengler Bernhard; Roempp Andreas

来源：Chromatographia, 2010, 72(9-10): 833-839.

DOI：10.1365/s10337-010-1743-2

• Column liquid chromatography
• Nano liquid chromatography-Fourier transform mass spectrometry
• Nuclear magnetic resonance
• 10-Deacthybaccatin III
• Taxine B and paclitaxel

In this work, an aqueous extraction method has been combined with a preparative LC for isolation of 10-deacetylbaccatin III (10-DAB) from leaves of Taxus baccata L. The extracted aqueous solution showed two major peaks in LC. Semi-preparative-LC was used for isolation of these peaks. Nano liquid chromatography-Fourier transform mass spectrometry and nuclear magnetic resonance showed that these peaks belong to taxine B and 10-DAB in the extracted aqueous solution. The residual sample of aqueous extraction solution was extracted again by MAE. The amount of paclitaxel in Taxus baccata L. was compared with and without aqueous extraction and results showed that paclitaxel was stable and had no significant difference in concentrations during this aqueous extraction process.

• 出版日期2010-11