Aggregation of nanoparticles in endosomes and lysosomes produces surface-enhanced Raman spectroscopy

作者：Lucas Leanne J; Chen Xiaoke K; Smith Aaron J; Korbelik Mladen; Zeng Haishan; Lee Patrick W K; Hewitt Kevin Cecil^*

来源：Journal of Nanophotonics, 2015, 9(1): 093094.

DOI：10.1117/1.JNP.9.093094

• surface-enhanced Raman spectroscopy
• epidermal growth factor receptor overexpression
• gold nanoparticles
• cancer imaging
• endosomes
• lysosomes

The purpose of this study was to explore the use of surface-enhanced Raman spectroscopy (SERS) to image the distribution of epidermal growth factor receptor (EGFR) in cells. To accomplish this task, 30-nm gold nanoparticles (AuNPs) tagged with antibodies to EGFR (10(12) per mL) were incubated with cells (10(6) per mL) of the A431 human epidermoid carcinoma and normal human bronchial epithelial cell lines. Using the 632.8-nm excitation line of a He-Ne laser, Raman spectroscopy measurements were performed using a point mapping scheme. Normal cells show little to no enhancement. SERS signals were observed inside the cytoplasm of A431 cells with an overall enhancement of 4 to 7 orders of magnitude. Raman intensity maps of the 1450 and 1583 cm(-1) peaks correlate well with the expected distribution of EGFR and AuNPs, aggregated following uptake by endosomes and lysosomes. Spectral features from tyrosine and tryptophan residues dominate the SERS signals.

• 出版日期2015-1-23