Determination and identification of antibiotic-resistant oral streptococci isolated from active dental infections in adults

作者：Pablo Loyola-Rodriguez Juan; Elena Ponce-Diaz Maria; Loyola-Leyva Alejandra; Garcia-Cortes Jose O.; Medina-Solis Carlo E.; Contreras-Ramire Azael A.; Serena-Gomez Eduardo

来源：Acta Odontologica Scandinavica, 2018, 76(4): 229-235.

DOI：10.1080/00016357.2017.1405463

• Antibiotic resistance bacteria
• amoxacillin
• Streptococcus mutans

Objective: To determine and identify antibiotic-resistant bacteria (ARB) of oral streptococci from active dental infections in adults and its association with age and gender.
Material and methods: This cross-sectional study included 59 subjects from 18 to 62 years old. Ninety-eighth samples obtained from the subjects were cultivated in agar plates containing antibiotics amoxicillin/clavulanic acid (A-CA), clindamycin, and moxifloxacin (concentrations of 16, 32 or 64 mu g/ml). PCR assay was performed to identify bacterial species.
Results: The bacterial species that showed more antibiotic-resistance (AR) was S. mutans (45.9%), followed by S. gordonii (21.6%), S. oralis (17.6%), S. sanguinis (9.5%), S. salivarius (5.4%) and S. sobrinus (0%). Moreover, clindamycin (59.4%) showed the highest frequency of AR. Moxifloxacin and A-CA showed an susceptibility >99.1%, while clindamycin showed the lowest efficacy (93.3%); there was a significant statistically difference (p<.01). The age group between 26 and 50 years old (32.2%) and females (28.8%) showed more multiresistance. Clindamycin showed a statistical difference (p <.05) when comparing groups by gender.
Conclusions: Clindamycin was the antibiotic with the highest frequency of ARB and lower bactericidal effect. Moxifloxacin and A-CA showed the highest efficacy and the lowest ARB frequency. Streptococcus mutans was the bacterial specie that showed an increased frequency of AR.

• 出版日期2018