Aspergillus flavus impairs antioxidative enzymes of Sternochetus mangzferae during mycosis

作者:Jayanthi Kamala*; Ayyasamy Arthikirubha; Kempraj Vivek; Aurade Ravindra M; Govindan Selvakumar; Verghese Abraham
来源:Journal of Invertebrate Pathology, 2015, 124: 73-77.
DOI:10.1016/j.jip.2014.11.004

摘要

Insects depend upon cuticular, humoral and cellular defenses to resist mycosis. However, entomopathogenic fungi through co-evolution have developed mechanisms to counter such defenses. Although a plethora of mechanisms of mycosis by entomopathogenic fungi are well-established, studies on the impairment of insects' antioxidative enzymes during mycosis remain elusive. Here, we used the interaction of Sternochetus mangiferae and its associated entomopathogenic fungus, Aspergillus flavus, as a model to validate our hypothesis. Uninfected insects were exposed to fungal spores for infection to occur. We observed symptoms of mycosis within 48 h of incubation period. Biochemical studies on antioxidative enzymes namely catalase, peroxidase and phenoloxidase, in infected and uninfected insects revealed decreased activity of these enzymes. It appears that A. flavus disables the host's antioxidative enzyme system that plays a crucial role in elimination of oxidative toxins produced during mycosis.

  • 出版日期2015-1