A new electrochemical method for DNA sequence detection with homogeneous hybridization based on host-guest recognition technology

作者：Fan H.; Xing R.; Xu Y.; Wang Q.; He P.^*; Fang Y.

来源：Electrochemistry Communications, 2010, 12(4): 501-504.

DOI：10.1016/j.elecom.2009.11.030

• Cyclodextrins
• DNA
• Double-labeled probe
• Electrochemical
• Homogeneous

This communication reports on a new electrochemical method to detect the hybridization specificity by using host-guest recognition technique. A hairpin DNA with dabcyl-labeled at its 3′ and NH2 group at 5′ terminal was combined with CdS nanoparticle to construct a double-labeled probe (DLP), which could selectively hybridize with its target DNA in homogeneous solution. A β-CD modified Poly(N-acetylaniline) glassy carbon electrode was used for capturing the dabcyl label in DLP. When without binding with target DNA, the DLP kept its stem-loop structure which shielded the dabcyl molecule due to the loop of the hairpin DNA and CdS nanoparticle blocking dabcyl enter into the cavity of these β-CD molecules on the electrode. However, in present of complementary sequence, the target-binding DLP was incorporated into double stranded DNA, causing the DLP's loop-stem structure opened and then the dabcyl was easily captured by the β-CD modified electrode. During electrochemical measurement, the signal from the dissolved Cd2+ was used for target DNA quantitative analysis.

• 出版日期2010
• 单位华东师范大学