Detection of the BCR-ABL fusion. Comparative analysis of six commercially available FISH fusion probes

作者：Knas T; Buck M; Stegelmann F; Moeller P; Barth T F

来源：Pathologe, 2015, 36(4): 372-384.

DOI：10.1007/s00292-015-0040-0

• BCR-ABL-FISH
• Ulm EDTA protocol
• Formalin fixation
• Bone marrow trephine biopsy
• Optimization

Chronic myeloid leukemia (CML) is diagnostically defined by the reciprocal translocation t(9;22)(q34;q11). This aberration can be detected by the BCR-ABL fluorescence in situ hybridization (FISH) technique. This article presents a comparative analysis of different commercially available FISH probes and different FISH protocols in order to optimize this technique on formalin-fixed and paraffin-embedded bone marrow trephine biopsies.

• 出版日期2015-7