An alpha-galactosidase from an acidophilic Bispora sp MEY-1 strain acts synergistically with beta-mannanase

作者：Wang, Hui; Luo, Huiying; Li, Jiang; Bai, Yingguo; Huang, Huoging; Shi, Pengjun; Fan, Yunliu; Yao, Bin^*

来源：Bioresource Technology, 2010, 101(21): 8376-8382.

DOI：10.1016/j.biortech.2010.06.045

• Acidophilic alpha-galactosidase
• Bispora sp MEY-1
• Synergistic enzyme action

An alpha-galactosidase gene (AgalB) was cloned from the acidophilic fungus Bispora sp. MEY-1 and expressed in Pichia pastoris. The deduced amino acid sequence showed highest identity (35%) to the alpha-galactosidase from Penicillium simplicissimum, belonging to the glycosyl hydrolase family 27. The purified recombinant alpha-galactosidase (r-AgalB) exhibited optimal activity at pH 3.5 and 55 degrees C, was stable at pH 2.2-8.0, and showed higher hydrolytic activity towards galactomannan polysaccharides (guar gum and locust bean gum) than toward small galacto-oligosaccharides (melibiose, raffinose and stachyose). A synergistic (3-fold) increase in guar gum hydrolysis was observed when beta-mannanase Man5A from Bispora sp. MEY-1 and r-AgalB were combined. Further, an increase in the reaction time from 5 h to 12 h or increase of the temperature from 37 degrees C to 55 degrees C enhanced guar gum degradation by the enzyme combination. These properties make r-AgalB a good candidate for extensive application in the pulp/paper, food, and feed industries.

• 出版日期2010-11
• 单位东华理工大学; 中国农业科学院