Use of Microsatellite and SNP Markers for Biotype Characterization in Hessian Fly

作者：Schemerhorn Brandon J; Crane Yan Ma; Cambron Sue E; Crane Charles F; Shukle Richard H

来源：Journal of Insect Science, 2015, 15(1): 158.

DOI：10.1093/jisesa/iev138

• Hessian fly
• biotype
• microsatellite
• single-nucleotide polymorphism

Exploration of the biotype structure of Hessian fly, Mayetiola destructor (Say) (Diptera: Cecidomyiidae), would improve our knowledge regarding variation in virulence phenotypes and difference in genetic background. Microsatellites (simple sequence repeats) and single-nucleotide polymorphisms (SNPs) are highly variable genetic markers that are widely used in population genetic studies. This study developed and tested a panel of 18 microsatellite and 22 SNP markers to investigate the genetic structure of nine Hessian fly biotypes: B, C, D, E, GP, L, O, vH9, and vH13. The simple sequence repeats were more polymorphic than the SNP markers, and their neighbor-joining trees differed in consequence. Microsatellites suggested a simple geographic association of related biotypes that did not progressively gain virulence with increasing genetic distance from a founder type. Use of the k-means clustering algorithm in the STRUCTURE program shows that the nine biotypes comprise six to eight populations that are related to geography or history within laboratory cultures.

• 出版日期2015-11-5