Limitations in SELDI-TOF MS whole serum proteomic profiling with IMAC surface to specifically detect colorectal cancer

作者：Wang, Qi; Shen, Jing; Li, Zhen-fu; Jie, Jian-zheng; Wang, Wen-yue; Wang, Jin; Zhang, Zhong-tao; Li, Zhi-xia; Yan, Li; Gu, Jin^*

来源：BMC Cancer, 2009, 9(1): 287.

DOI：10.1186/1471-2407-9-287

• PROSTATE-CANCER
• MASS-SPECTROMETRY
• HEPATOCELLULAR-CARCINOMA
• CELL CARCINOMA
• OVARIAN-CANCER
• COLON-CANCER
• STOOL DNA
• PATTERN
• IDENTIFICATION
• BIOMARKERS

Background: Surface enhanced laser desorption and ionization time-of-flight mass spectrometry (SELDI-TOF-MS) analysis on serum samples was reported to be able to detect colorectal cancer (CRC) from normal or control patients. We carried out a validation study of a SELDI-TOF MS approach with IMAC surface sample processing to identify CRC. @@@ Methods: A retrospective cohort of 338 serum samples including 154 CRCs, 67 control cancers and 117 non-cancerous conditions was profiled using SELDI-TOF-MS. @@@ Results: No CRC "specific" classifier was found. However, a classifier consisting of two protein peaks separates cancer from non-cancerous conditions with high accuracy. @@@ Conclusion: In this study, the SELDI-TOF-MS-based protein expression profiling approach did not perform to identify CRC. However, this technique is promising in distinguishing patients with cancer from a non-cancerous population; it may be useful for monitoring recurrence of CRC after treatment.

• 出版日期2009-8-19
• 单位北京大学; 中日友好医院; 北京市肿瘤防治研究所; 华中科技大学