A stuttering start to plant DNA barcoding: microsatellites present a previously overlooked problem in non-coding plastid regions

作者：Devey Dion S^*; Chase Mark W; Clarkson James J

来源：Taxon, 2009, 58(1): 7-15.

DOI：10.1002/tax.581003

• atpF-H
• automated-barcoding
• DNA microsatellites
• electropherograms
• non-coding plastid DNA
• psbA-trnH

Plastid microsatellites are simple mono/dinucleotide repeats that can cause in-vitro taq polymerase errors leading to 'stutter' peaks that reduce the clarity and reliability of electropherograms. As an efficient and automated barcoding protocol requires high-quality raw data, this paper investigates the suitability of the psbA-trnH and atpF-H regions of the plastid genome for automated barcoding purposes. Amplification of 92 individuals from species pairs and triplets was undertaken. Of these, 59% of atpF-H species samples and 36% of psbA-trnH species samples showed evidence of rnicrosatellite-induced errors in electropherogram base calling. The downstream necessity of human intervention renders these non-coding regions sub-optimal for barcoding purposes.

• 出版日期2009-2