科研之友
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摘要
A simple, rapid and sensitive ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method is described for determination of levofloxacin in human plasma samples using enrofloxacin as the internal standard (IS) from bioequivalence assays. Sample preparation was accomplished through protein precipitation with acetonitrile, and chromatographic separation was performed on an Acquity BEH C18 column (2.1 mm x 50 mm, 1.7 mu m) with gradient profile at a flow of 0.45 mL/min. Mass spectrometric analysis was performed using a QTrap5500 mass spectrometer coupled with an electro-spray ionization (ESI) source in the positive ion mode. The MRM transition of 362.7 -> 261.2 was used to quantify for levofloxacin. The linearity of this method was found to be within the concentration range of 10-2500 ng/mL for levofloxacin in human plasma. Only 1.0 min was needed for an analytical run. The method was applied to a bioequivalence study of two drug products containing levofloxacin in human plasma samples.
