Accelerated Neuritogenesis and Maturation of Primary Spinal Motor Neurons in Response to Nanofibers

作者：Gertz Caitlyn C; Leach Michelle K; Birrell Lisa K; Martin David C; Feldman Eva L; Corey Joseph M^*

来源：Developmental Neurobiology, 2010, 70(8): 589-603.

DOI：10.1002/dneu.20792

• motor neuron
• neuritogenesis
• neuronal polarity
• nanofibers
• guidance
• lamellipdodia

Neuritogenesis, neuronal polarity formation, and maturation of axons and dendrites are strongly influenced by both biochemical and topographical extracellular components. The aim of this study was to elucidate the effects of polylactic acid electrospun fiber topography on primary motor neuron development, because regeneration of motor axons is extremely limited in the central nervous system and could potentially benefit from the implementation of a synthetic scaffold to encourage regrowth. In this analysis, we found that both aligned and randomly oriented submicron fibers significantly accelerated the processes of neuritogenesis and polarity formation of individual cultured motor neurons compared to flat polymer films and glass controls, likely due to restricted lamellipodia formation observed on fibers. In contrast, dendritic Maturation and soma spreading were inhibited on fiber substrates after 2 days in vitro. This study is the first to examine the effects of electrospun fiber topography on motor neuron neuritogenesis and polarity formation. Aligned nanofibers were shown to affect the directionality and timing of motor neuron development, providing further evidence for the effective use of electrospun scaffolds in neural regeneration applications.

• 出版日期2010-7