科研之友
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摘要
Simple, accurate, rapid, and sensitive spectrofluorimetric methods for the determination of levosulpiride in pharmaceutical formulation were developed utilizing its fluorescence reaction with Fe(3+) (method A) and Al(3+) (method B). The calibration curves were found to be linear in the concentration range 0.239-3.44 mu g/mL and 0.310-2.730 mu g/mL with limit of detection 0.005 mu g/mL and 0.0032 mu g/mL, respectively, for method A and method B. The reaction conditions were studied and optimized. In addition, the complexation of Mg(2+) and Ca(2+) was also studied. In all cases, an enhancement in fluorescence emission of levosulpiride upon formation of complex with metal ions was observed. A 2: 1 (drug: metal) stoichiometry for all the complexes was established. Benesi-Hildebrand method was applied for calculation of association constant at 25 and 35A degrees C. The thermodynamic parameters obtained in this study revealed that the interaction process was spontaneous and mainly Delta S-driven.
- 出版日期2011-7
