A DQA1 ALLELE IS STRONGLY ASSOCIATED WITH IDIOPATHIC MEMBRANOUS NEPHROPATHY

作者：VAUGHAN RW; DEMAINE AG; WELSH KI

来源：Tissue Antigens, 1989, 34(5): 261-269.

DOI：10.1111/j.1399-0039.1989.tb01741.x

<jats:p>The high molecular weight genomic DNA from 31 patients with idiopathic membranous nephropathy (IMN) has been digested with a restriction endonuclease Taq I, electrophoresed, blotted and hybridised with probes to the HLA‐DRB, ‐DQA, ‐DQB, ‐DPA and ‐DPB genes. The restriction endonuclease Msp I was also used with the HLA‐DPA and ‐DPB probes. The resulting restriction fragment length polymorphism (RFLP) has been analysed and compared with 55 controls treated in the same way. There was a significant increase in the DRB restriction fragments associated with HLA‐DR3 (Fisher's p = 0.011), in particular with a sub‐division of DR3 (Fisher's p = 0.0038). These results confirm at the DNA level serological correlations observed for IMN. A 4.5 Kb DQA RFLP was significantly raised in IMN patients (Fisher's p = 0.0002) and is proposed as a major disease susceptibility factor.</jats:p>

• 出版日期1989-11