Arabinogalactan from edible jambo fruit induces different responses on cytokine secretion by THP-1 macrophages in the absence and presence of proinflammatory stimulus

作者：Tamiello Camila Silva; do Nascimento Georgia Erdmann; Iacomini Marcello; Cordeiro Lucimara M C^*

来源：International Journal of Biological Macromolecules, 2018, 107: 35-41.

DOI：10.1016/j.ijbiomac.2017.08.148

• Jambo type II arabinogalactan
• THP-1 cells
• Cytokine secrection

Syzygium jambos is an Indo-Malaian found in many tropical countries and it is mainly composed of carbohydrates. Fraction PF-WSP and SF-WSP were obtained by aqueous extraction followed by Fehling's treatment. Monosaccharide analysis showed that fraction PF-WSP has a high content of uronic acids (90%) and fraction SF-WSP presented mainly galactose (39.1%) and arabinose (34.2%), as neutral sugars and 9% of galacturonic acid. The presence of type II arabinogalactan in SF-WSP was evidenced by methylation analysis and C-13/H-1 HSQC NMR experiments. Immunomodulating properties of SF-WSP was investigated. It decreases THP-1 macrophage viability at the highest concentration tested (200 mu g/mL). We then tested non-cytotoxic concentrations of SF-WSP on THP-1 cytokine production in the presence and absence of LPS. The results showed that SF-WSP increased TNF-alpha, IL-1 beta and IL-10 secretion in a concentration-dependent manner as well as attenuated the inflammatory response induced by LPS at the highest concentration (100 mu g/mL). These results contribute to elucidate the effects of fruit pectic polysaccharides on immune cells.

• 出版日期2018-2