Antioxidant and anticholinesterase active constituents from Micromeria cilicica by radical-scavenging activity-guided fractionation

作者：Ozturk Mehmet; Kolak Ufuk; Topcu Gulacti^*; Oksuz Sevil; Choudhary M Iqbal

来源：Food Chemistry, 2011, 126(1): 31-38.

DOI：10.1016/j.foodchem.2010.10.050

• Lamiaceae
• Micromeria cilicica
• Antioxidant activity
• Anticholinesterase activity
• Piperitone 7-O-beta-D-glucoside
• Isothymonin 4 '-methyl ether

From the acetone extract of Micromeria cilicica, two new (1 and 2) and five known (3-7) compounds were obtained through radical-scavenging activity guided isolation. Structures of the compounds were identified as piperitone 7-O-beta-D-glucoside (1), isothymonin 4'-methyl ether (2), sudachitin (3), isomucronulatol (4), rutin (5), ursolic acid (6) and saccharose (7), based on UV, 1D-and 2D-NMR and mass spectroscopic techniques. The antioxidant potentials of the extract and the isolated compounds were established by using three radical-scavenging assays, namely, DPPH. scavenging, O(2)(center dot-) scavenging and ABTS(center dot+) scavenging, besides beta-carotene bleaching assay. Particularly, the acetone extract showed a strong inhibition of lipid peroxidation by beta-carotene bleaching assay, with a result close to that of (+)-catechin. Among the pure compounds, rutin (5) showed the strongest lipid peroxidation inhibition and antiradical activity while both sudachitin (3) and isomucronulatol (4) exhibited noticeable ABTS(center dot+) scavenging activity. The anticholinesterase activity of the compounds (1-7) was also determined. Against acetylcholinesterase, they exhibited weak inhibition while compounds 3, 4 and 6 exhibited moderate inhibition against butyrylcholinesterase.

• 出版日期2011-5-1