摘要
Little is known about the mechanism of drought tolerance in rapeseed (Brassica napus L.). In this study, different morphological and physiological responses to drought stress were studied in three rapeseed cultivars. For the cultivar 2AF009 with high drought tolerance, comparative proteomic analyses were conducted to determine the molecular mechanism behind. Approximately 138 differentially abundant proteins (DAPs) and 1232 phosphoproteins containing 4469 phosphopeptides were identified. Furthermore, 337 phosphoproteins containing 547 phosphorylation sites demonstrated significant changes. These drought-responsive DAPs and phosphoproteins were mainly involved in signal transduction, photosynthesis, and glutathione-ascorbate metabolism. Notably, 9 DAPs were also identified as drought-responsive phosphoproteins, especially beta carbonic anhydrase 1 (beta CA1), which was represented by eight distinct protein spots with different abundant levels during drought stress. Tyr207 phosphorylated site of beta CA1 was down-regulated at the phosphorylation level during drought stress, which was also located in the substrate-binding active region of three-dimensional (3D) structure. Moreover, drought stress inhibited CA activity. We concluded that Tyr207 was the most likely phosphorylation target affecting the enzyme activity, and phosphorylation of beta CA1 might be important for the response to drought stress in rapeseed. The study provided a new clue for the drought tolerance mechanism in B. napus.
- 出版日期2016-12-14
- 单位中国农业大学; 中国农业科学院; 中国热带农业科学院; 农业生物技术国家重点实验室; 中国热带农业科学院椰子研究所; 华中农业大学